The sequence shown corresponds to allele CYP21A2*1B. 1989 Sep;3(9):1399-408. Functional analysis variants . The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. Search for other works by this author on: Copyright © 1989 by The Endocrine Society, Editorial: Final Musings on the Impact of Molecular Endocrinology, Editorial: Reflections on the Impact of Molecular Endocrinology on a Scientific Career, The Journal of Clinical Endocrinology & Metabolism, Receive exclusive offers and updates from Oxford Academic, Gene Structure Of Chum Salmon Somatolactin, A Presumed Pituitary Hormone Of The Growth Hormone/Prolactin Family, Definition of the Human Androgen Receptor Gene Structure Permits the Identification of Mutations that Cause Androgen Resistance: Premature Termination of the Receptor Protein at Amino Acid Residue 588 Causes Complete Androgen Resistance, The Gene Structure of Rat Androgen-Binding Protein: Identification of Potential Regulatory Deoxyribonucleic Acid Elements of a Follicle-Stimulating Hormone-Regulated Protein, Tryptamine Serves As a Proligand of the AhR Transcriptional Pathway Whose Activation Is Dependent of Monoamine Oxidases. These sequences represent the protein coding region of the CYP1A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. The Oncologist. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Between the human CYP1A2 and CYP1A1 (cytochrome P1450) genes, exons 2, 4, 6, and especially 5 are strikingly conserved in both nucleotide similarity and total number of bases. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:9435160, PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:19965576). The following CYP1A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). Find diseases associated with this biological target and compounds tested against it in bioassay experiments. The transcriptional start site was determined by both primer extension and S1 mapping. This gene was present in the common ancestor of eukaryotes. The CYP1A2*1F polymorphism is located in a non-coding region of the gene, but may result in differential binding of regulatory proteins to the surrounding sequence and alter CYP1A2 expression levels. Rfam classification and
PrimePCR™ SYBR® Green Assay: CYP1A2, Human cytochrome P450, family 1, subfamily A, polypeptide 2 Assay Type: SYBR® Green Assay Design: Intron-spanning Application: Gene Expression Unique Assay ID: qHsaCID0015160 Info: Same primer pair as used in probe assay qHsaCIP0029751 The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The CYP1A2 gene encodes a P450 enzyme involved in O-deethylation of phenacetin. [provided by RefSeq, Jul 2008] Associated conditions. Various DNA fragments from the CYP1A2 gene were inserted into the ClaI site of pMC53c which contains the CYP1A1 promoter , and designated pMCD-x or pMCD-xr (x indicates the length of the 5 ′ upstream sequence of the CYP1A2 gene from the transcription-initiation site, and r indicates reverse orientation). The canonical 5-bp box (CACGC), found upstream of all mammalian CYP1A1 genes to date and believed to interact with the inducer. Gene target information for CYP1A2 - cytochrome P450 family 1 subfamily A member 2 (human). They assigned the gene to chromosome 15. We performed a meta-analysis of four genome-wide association studies of coffee consumption among coffee drinkers from Iceland (n = 268 … Sequence variants at CYP1A1-CYP1A2 and AHR associate with coffee consumption Hum Mol Genet. Among 12 human liver samples, striking differences (> 15-fold) in the 3.3-kilobase 1A2 mRNA levels were seen. Cytochrome P450 1A2 (CYP1A2) is one of the CYP450 mixed-function oxidase system that is of clinical importance due to the large number of drug interactions associated with its induction and inhibition. Cyp1a2 (untagged ORF) - Rat cytochrome P450, family 1, subfamily a, polypeptide 2 (Cyp1a2), (10 ug) Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. [provided by RefSeq, Jul 2008] [provided by RefSeq, Jul 2008] Mouse(13077) cytochrome P450, family 1, subfamily a, polypeptide 2 CYP1A2 (Cytochrome P450 Family 1 Subfamily A Member 2) is a Protein Coding gene. Interacts with PGRMC1; the interaction requires PGRMC1 homodimerization. These mutations are displayed at the amino acid level across the full length of the gene by default. CYP1A2 (Cytochrome P450 Family 1 Subfamily A Member 2) is a Protein Coding gene. An important paralog of this gene is CYP1A1. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other … The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. View mouse Cyp1a2 Chr9:57676937-57683655 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression DNA sequence analysis was performed on an ABI 373A instrument using a Taq DyeDeoxy Cycle Sequencing Kit TM (Perkin Elmer, Weiterstadt, Germany). Influence of genetic polymorphisms, smoking, gender and age on CYP1A2 activity in a Turkish population. To date, 16 alleles (and many more variants) of the CYP1A2 gene have been identified (see http://www.imm.ki.se/CYPalleles/cyp1a2.htm). Gene Names: Gene Aliases: RefSeq Accessions: SNP IDs (if applicable): Mature Names (if applicable): Hs00342154_CE details, Hs00342154_CE search: Human: cytochrome P450 family 1 subfamily A member 2: CP12;P3-450;P450(PA) NM_000761.4 However, little else is known of the mechanisms involved in Cyp1a2 gene regulation, in part due to the lack of an established cell line expressing CYP1A2. lentivirus, AAV, adenovirus), VectorBuilder Virus packaging for CYP1A2 (ie. Restrict the view to a region of the gene by dragging across the histogram to highlight the region of interest, or by using the sliders in the filters panel to the left. The coding region starts at nucleotide 10 of exon 2. MARIE-GENICA Consortium on Genetic Susceptibility for Menopausal Hormone Therapy Related Breast Cancer Risk. CircDUSP16 Contributes to Cell Development in Esophageal Squamous Cell Carcinoma by Regulating miR-497-5p/TKTL1 Axis. Functional Associations. knockout, knockin, CRISPRa, CRISPRi), VectorBuilder Virus packaging for CYP1A2 CRISPR vectors (ie. Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer (PubMed:19965576). CYP1A2 enzyme is a 515-residue protein with a molecular mass of 58294 Da [ 3 ]. Plays a role in the oxidative metabolism of xenobiotics. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin (PubMed:14725854). You can see various sequences for this gene: cDNA (ENST00000343932.4) Protein (CYP1A2) Transcript and protein aligned (ENST00000343932.4+CYP1A2) Gene fusions No fusions involving CYP1A2 Drug sensitivity data n/a We wanted to determine how much of the variability of CYP1A2 activity is explained by a newly discovered gene polymorphism in intron 1. New alternative splicing variants of the ATXN2 transcript. Lenti ORF clone of Human cytochrome P450, family 1, subfamily A, polypeptide 2 (CYP1A2), Myc-DDK-tagged CYP1A2 ist am oxidativen Metabolismus einer Reihe von Arzneistoffen und Umwelttoxinen beteiligt. The CYP2C9 enzyme breaks down (metabolizes) compounds including steroid hormones and fatty acids. Results Out of the total … The gene view histogram is a graphical view of mutations across CYP1A1. [PubMed:2575218] Jaiswal AK, Nebert DW, McBride OW, Gonzalez FJ: Human P(3)450: cDNA and complete protein sequence, repetitive Alu sequences … AHR is known to induce CYP1A1 and CYP1A2 by binding to the DNA in the region between those two genes. Another is the AHR gene, which controls when and how the CYP1A2 gene is switched on and off (2). The human CYP1A2 gene is located on chromosome 15 and contains seven exons. Hildebrand et al. [provided by RefSeq, Jul 2008] Mouse(13077) cytochrome P450, family 1, subfamily a, polypeptide 2 ( RefSeq ) able to metabolize some PAHs to carcinogenic intermediates and 1... Kilobases, comprising seven exons and six introns, the first exon a. Cyp1A2 - cytochrome P450 family of proteins, which is encoded by the open reading frame ORF!, Beijnen JH, Schellens JH ( 2006 ) metabolizes cholesterol toward 25-hydroxycholesterol, a regulator... 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